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. 2019 Apr 12;9(8):2209–2223. doi: 10.7150/thno.30726

Figure 4.

Figure 4

LL-37 activates Wnt/β-catenin signaling to promote growth of lung tumors. (A) Immunohistochemical analysis of the expression of unphosphorylated β-catenin in WT and Cramp-/- mice injected with 1×105 LLC cells via the tail vein. Scale bar, 100 μm. (B) Immunoblotting analysis in isolated tumors from WT and Cramp-/- mice injected with 1×105 LLC cells via the tail vein. (C) Immunohistochemical analysis of the expression of unphosphorylated β-catenin and LL-37 in serial sections of NSCLC tumor specimens. Scale bar, 100 μm. (D) Immunofluorescence staining shows the subcellular localization of unphosphorylated β-catenin and actin (phalloidin) in A549 cells. Scale bar, 20 μm. (E) Western blotting analysis of unphosphorylated β-catenin, total β-catenin, phosphorylated Gsk3β, pan Gsk3β, phosphorylated Akt, pan Akt, Cyclin D1, c-myc, TTP, and β-actin in A549 cells after stimulation with LL-37 for indicated time periods.