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Western blot analysis (A), 2D (B) and 3D (C) morphologies of CD166 expression at different time points for both aligned and random co-culture groups. The CD166 tracks were identified in aligned ECM/hMSC/EC groups, as identified by yellow arrows (B) The CD166 was concentrated at the interface between hMSCs and ECs in the aligned groups and guided the microvessels progression, while scattered CD166 expression was observed in random groups. (*p < 0.05, **p < 0.01)
