Figure 6.

Exosome uptake quantified by flow cytometry. Murine osteosarcoma K7M2 cells were incubated with unlabeled mesenchymal stromal cell-derived exosomes (Control) or Rhodamine B (RhB)- labeled exosomes (Exo-RhB) and the time-dependent uptake was measured by flow cytometry (10000 events per histogram). (A) Flow cytometry histograms indicate RhB-positive events (P2) for unlabeled exosomes (Control), or after 6 h, 12 h, 24 h, and 48 h of incubation with Exo-RhB. (B) Integrated fluorescence intensity (RhB intensity) of Exo-RhB within K7M2 cells. Note that RhB intensity with K7M2 cells increases over the first 24 h. Data shown is median area under the curve ± 1 standard deviation.