Skip to main content
. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Appl Toxicol. 2019 Jan 28;39(6):888–898. doi: 10.1002/jat.3777

Figure 2:

Figure 2:

The effect of e-liquids on cell viability. Saos-2 cells were treated for 48 h with e-liquid containing a final nicotine concentration of 0.001, 0.01, 0.1, 0.5, or 1.0 mg/ml or an equivalent volume of e-liquid without nicotine at 0.004% 0.04%, 0.4%, 2.0% or 4.0%, respectively. Cell viability was determined using the MTT assay. Results are expressed as percent cell viability. Each bar represents the mean ± SEM of at least 3 independent experiments.* denotes a significant difference from culture medium only control (p <0.05). † denotes significant difference from the matched control without nicotine (p <0.05). The e-liquids tested were (A) Vapor Emporium brand, (B-E) Lotus brand, (F-J) Mister-E-Liquid brand.