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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Appl Toxicol. 2019 Jan 28;39(6):888–898. doi: 10.1002/jat.3777

Figure 4:

Figure 4:

Immunofluorescence detection of cytosolic collagen type I protein. MG-63 cells were treated for 48 h with Lotus brand Mango Blast or Mister-E-liquid Clear (Flavorless). A panel of images representative of one experiment with culture medium control, 0.4% e-liquid treatment without nicotine, and 0.4% e-liquid treatment containing 0.1mg/ml nicotine. Images were analyzed and quantified as the percent area of the image within the intensity threshold using ImageJ software. The values presented are the mean ± SEM of 3 independent experiments. * denotes significant difference from culture medium only control (p <0.05).