| Primer seauence (5’ to 3’) for aRT-PCR | Temp (°C) |
| RUNX2: TAT GGC ACT TCG TCA GGA TCC | 64°C |
| AAT AGC GTG CTG CCA TTC G | |
| Collal: AAC ATG ACC AAA AAC CAA AAG TG | 63°C |
| CAT TGT TTC CTG TGT CTT CTG G | |
| GAPDH: CTC TGC TCC TCC TGT TCG AC | 53°C |
| TTA AAA GCA GCC CTG GTG AC |
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