TABLE 1: E-liquid Product Information and Cytotoxicity.
EC50 values for e-liquids from Vapor Emporium, Lotus, Mister-E-liquid, and Vape Dudes brands. For each e-liquid, its brand, name, PG/VG ratio, and stock concentrations of nicotine are identified. MG-63 and Saos-2 cells were treated for 48 h with e-liquid containing a final nicotine concentration of 0.001, 0.01, 0.1, 0.5, or 1.0 mg/ml or an equivalent volume of e-liquid without nicotine at 0.004% 0.04%, 0.4%, 2.0% and 4.0%, respectively. Each EC50 value (expressed as % volume of e-liquid) was calculated using a linear model of the compiled cell viability data, with or without nicotine, for each cell line.
| Flavor | Brand | Name | PG/VG | Stock Concentration Nicotine (mg/ml) | MG-63 EC50 (% volume) | Saos-2 EC50 (% volume) |
|---|---|---|---|---|---|---|
| Flavorless | Vapor Emporium | Flavorless | Not Reported | 0 | 3.10 | 3.10 |
| 24 | 2.38 | 3.26 | ||||
| Mister E-liquid | Clear | 50/50 | 0 | 6.19 | 3.27 | |
| 24 | 4.71 | 2.38 | ||||
| Vape Dudes | Flavorless | 50/50 | 0 | 5.40 | 3.92 | |
| 24 | 7.30 | 3.14 | ||||
| Watermelon | Lotus | Sweet Melon | Not Reported | 0 | 2.68 | 2.12 |
| 24 | 2.01 | 1.62 | ||||
| Mister E-liquid | Watermelon | 50/50 | 0 | 3.47 | 3.32 | |
| 24 | 2.93 | 3.21 | ||||
| Vape Dudes | Watermelon Drip | 50/50 | 0 | 2.71 | 2.63 | |
| 24 | 2.13 | 2.04 | ||||
| Mango | Lotus | Mango Blast | Not Reported | 0 | 2.41 | 2.10 |
| 24 | 2.76 | 2.18 | ||||
| Mixed Fruits | Lotus | XXX Berry | Not Reported | 0 | 1.93 | 1.64 |
| 24 | 2.07 | 1.63 | ||||
| Mister E-liquid | Heartbreaker | 50/50 | 0 | 2.57 | 2.08 | |
| 24 | 2.74 | 2.64 | ||||
| Vape Dudes | Possum Sauce | 50/50 | 0 | 2.73 | 2.60 | |
| 24 | 2.51 | 2.28 | ||||
| Coffee | Lotus | Irish Latte | Not Reported | 0 | 3.20 | 2.49 |
| 24 | 2.30 | 1.98 | ||||
| Mister E-liquid | G.T.F.O. | 50/50 | 0 | 2.40 | 2.36 | |
| 24 | 1.65 | 1.50 | ||||
| Vape Dudes | Irish Coffee | 50/50 | 0 | 6.67 | 2.96 | |
| 24 | 5.92 | 2.93 | ||||
| Apple Pie | Mister E-liquid | Gran-E’s Apple Pie | 50/50 | 0 | 2.11 | 1.78 |
| 24 | 1.77 | 1.01 | ||||
| Vape Dudes | Apple Pie | 50/50 | 0 | 3.02 | 2.62 | |
| 24 | 2.63 | 2.41 | ||||
| Menthol & Watermelon | Vape Dudes | Watermelon ICE | 50/50 | 0 | 2.12 | 2.38 |
| 24 | 2.00 | 1.68 | ||||
| Menthol | Lotus | Menthol | Not Reported | 0 | 1.75 | 1.99 |
| 24 | 1.50 | 2.21 | ||||
| Mister E-liquid | Mister E’s Menthol | 50/50 | 0 | 2.15 | 2.32 | |
| 24 | 1.62 | 1.43 | ||||
| Vape Dudes | ICE ICE | 50/50 | 0 | 1.86 | 2.12 | |
| 24 | 1.93 | 2.19 | ||||
| Hot Cinnamon | Lotus | Fireball | Not Reported | 0 | 1.23 | <0.004 |
| 24 | 0.97 | <0.004 | ||||
| Mister E-liquid | Napalm | 50/50 | 0 | 1.21 | <0.004 | |
| 24 | 0.54 | <0.004 | ||||
| Vape Dudes | Cinn Candy | 50/50 | 0 | 1.76 | <0.004 | |
| 24 | 1.56 | <0.004 | ||||
| Menthol & Cinnamon | Vape Dudes | FIRE & ICE | 50/50 | 0 | 1.66 | 2.02 |
| 24 | 0.97 | 0.01 |