Figure 5. Skin-homing memory cells are decreased in Itgb6^−/−Itgb8^ΔKC mice.

(A and B) Splenic B8R⁺ cells from WT or Itgb6^−/−Itgb8^ΔKC mice were analyzed for memory subsets based on expression of CXCR3 and CX3XR1 by flow cytometry at day 68 post skin vaccinia virus (VV) infection: CXCR3⁺CX3CR1⁻ Tcm cells, CXCR3⁺CX3CR1^int Tpm cells and CXCR3⁻CX3CR1^high Tem cells. (A) Representative flow cytometry plots gated on B8R⁺ cells and the (B, left panel) frequency and (B, right panel) number of each memory subset of B8R⁺ cells are shown. (C and D) E-selectin or P-selectin binding (ESL⁺ or PSL⁺ respectively) of splenic B8R⁺ cells were analyzed by flow cytometry following skin VV infection. Representative flow plots (C) and the frequency of ESL⁺ (top panels) or PSL⁺ (bottom panels) B8R⁺ cells day 7 and 68 post infection are shown. (D) Frequency of ESL⁺ (top panels) or PSL⁺ (bottom panels) among each subset of memory B8R⁺ cells in the spleen are shown. Data are representative of two separate experiments. Each dot represents an individual mouse. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. See also Figure S6.