Table-4.
Calculated “two pore theory” related parameters for IgG (150 kDa), F(ab)2 (100 kDa), Minibody (80 kDa), scFv2(55 kDa), Fab and Diabody (50 kDa), scFv (27 kDa), and nanobody (13 kDa).
| Parameter | Description | Nanobody (13 kDa) | scFv (27 kDa) | Fab and Diabody (50 kDa) | scFv2 (55 kDa) | Minibody (80kDa) | F(ab)2 (100 kDa) | IgG (150 kDa) |
|---|---|---|---|---|---|---|---|---|
| ae (nm) | Stokes-Einstein radius | 1.87 | 2.48 | 3.15 | 3.26 | 3.77 | 4.11 | 4.81 |
| ae/rS | relative ratio between protein size and small pore size | 0.421 | 0.559 | 0.709 | 0.735 | 0.849 | 0.926 | 1.083 |
| ae/rL | relative ratio between protein size and large pore size | 0.0819 | 0.109 | 0.138 | 0.143 | 0.165 | 0.18 | 0.21 |
| σS | Small pore vascular reflection coefficient | 0.495 | 0.712 | 0.885 | 0.906 | 0.965 | 0.984 | 0.998 |
| σL | Large pore vascular reflection coefficient | 0.0312 | 0.0526 | 0.0819 | 0.0877 | 0.115 | 0.135 | 0.18 |
| A/A0S | Fractional accessible pore size of small pore | 8.08E-02 | 2.50E-02 | 3.72E-03 | 2.45E-03 | 3.09E-04 | 5.87E-05 | 9.28E-07 |
| A/A0L | Fractional accessible pore size of large pore | 0.693 | 0.599 | 0.533 | 0.522 | 0.469 | 0.431 | 0.349 |
| PsS_heart (L/hr) | Permeability-surface area product of small pore (heart as an example) | 2.02E-03 | 4.73E-04 | 5.53E-05 | 3.52E-05 | 3.83E-06 | 6.69E-07 | 9.04E-09 |
| PsL_heart (L/hr) | Permeability-surface area product of large pore (heart as an example) | 2.87E-05 | 1.87E-05 | 1.31E-05 | 1.24E-05 | 9.64E-06 | 8.13E-06 | 5.63E-06 |
| Jiso_heart (L/hr) | Circular isogravimetric flow (heart as an example) | 2.77E-05 | 2.77E-05 | 2.77E-05 | 2.77E-05 | 2.77E-05 | 2.77E-05 | 2.77E-05 |
| PeS | Peclet number of small pore | 0.0105 | 0.0257 | 0.0876 | 0.113 | 0.381 | 0.981 | 9.82 |
| PeL | Peclet number of large pore | 1.04 | 1.59 | 2.15 | 2.27 | 2.83 | 3.28 | 4.48 |
| θ | Glomerular sieving coefficient | 0.667 | 0.403 | 0.131 | 0.0986 | 0.022 | 0.00703 | 0.0011 |