Figure 1: Infection with LCMV clone 13 leads to transient cachexia.

a) Body weight kinetics for C57BL6/J wild-type mice infected with 2×10⁶ FFU of LCMV-Cl13, in comparison with pair-fed mice (n=5). b-c) Food intake (b) and water intake (c) of LCMV-infected mice in comparison with uninfected controls. (n=10) (p-values: ****<0.0001, two-way ANOVA), Data are representative of three independent experiments for a) and b), c) represents a single experiment. d) Activity, oxygen consumption and respiratory exchange ratio (RER) in LCMV-infected mice compared to uninfected controls (n=10). Data shown represent the average of day 6, 7 and 8 post-infection (p-values: ****<0.0001) from a single experiment. e) Percentage of tissue weight normalized to body weight prior to infection in inguinal, gonadal and interscapular brown adipose tissue (p-values: **0.002, ***0.0006, *0.0172, ***0.0009 unpaired two tailed Student’s t-test), as well as quadriceps, gastrocnemius and soleus muscles (p-values: *0.039, **0.0035, ***0.0001 unpaired two tailed Student’s t-test) at day 7 for pair-fed uninfected mice and day 6 and 8 post-infection for LCMV-infected mice (n=5). f) Body composition as measured in live un-anesthetized LCMV-infected mice using EchoMRI (n=5). e-f) shows representative data of two independent experiments g) Body weight kinetics in LCMV-infected mice supplemented with the equivalent of 1kcal of indicated diet through oral gavage daily between day 4 and 7 post-infection (n=4). (p-values: ***0.0003 two-way ANOVA, Bonferroni correction). Data are representative of three independent experiments. All data shows mean ± s.e.m.