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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Mech Dev. 2019 Apr 8;157:1–9. doi: 10.1016/j.mod.2019.04.001

Figure 3:

Figure 3:

WT pks1 nucleic acid rescues otolith formation in csr, nco, and vns. (A) Normal frequencies of mutant phenotypes in each uninjected strain. All four pairings follow homozygous recessive mode of inheritance. (B) Results of injected embryos show that Japanese medaka pks1 mRNA (300 pg) rescues both csr and nco mutants and pks1 DNA (20 pg) rescues vns mutants. (*, p < 0.0001, paired t-test)(**, p < 0.0032, paired t-test)(***, p = 0.0001, paired t-test),. Site-directed mutagenesis was used to introduce a conserved mutation in csr (A911P) into the Japanese medaka construct (L905P) (C) Injection of pks1L905P (300 pg) fails to rescue csr or nco mutant phenotypes.