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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Acta Biomater. 2018 Nov 22;95:236–244. doi: 10.1016/j.actbio.2018.11.024

Figure 5. Ethanol conditioning enhances angiogenic lncRNA cargo in HDMEC-derived EVs.

Figure 5.

Expression levels of lncRNAs HOTAIR and MALAT1 were assessed by qPCR in EVs derived from HDMECs cultured with or without 100 mM ethanol for 24 h in each of the culture conditions. B-actin levels were assessed as a control. Data are shown as fold change of expression levels in ethanol treated group vs. expression levels in untreated group. No statistical significance was observed between the treated and untreated groups for B-actin. Significant variance was calculated for HOTAIR and MALAT1 for all three indicated culture conditions between ethanol treated and untreated groups. Data represent three independent experiments, each performed in triplicate (n=3). Statistical significance was calculated using two-way ANOVA with Bonferroni’s multiple comparison test (*p<0.05, **p<0.01, ***p<0.001).