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. 2019 May 22;9:7717. doi: 10.1038/s41598-019-44147-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DHE fluorescence in frontal cortex indicating superoxide production. (A) Low level of flurescence intensity in control tissue, basal level of superoxide. (B) Levels of superoxide robustly increase four hours following blast exposure, (C,D). Supreoxide is brought back down to basal levels with pretreatment of apocynin Quantitation of DHE fluorescence intensity for the three aforementioned groups. Scale bar = 1 mm. **Indicates a difference between control ((n = 4) and blast groups (n = 4) as well as blast + apocynin group (n = 4) with a statistical significance of p < 0.01.