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. 2019 May 22;9:7696. doi: 10.1038/s41598-019-42773-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptional validation of differentially expressed genes by qRT-PCR based on CD271 status and normalization against 18 s RNA using the ΔCT method. The fold change for a transcript of interest was determined by taking the ratio of normalized gene expression between CD271⁺ and CD271⁻ cell populations for each patient sample. In brief, differential gene expression was determined by calculating the fold change of any gene of interest (GOI) = 2^−ΔΔCT, where ΔCT(GOI) = (CT(GOI) − CT(18 s) and ΔΔCT(GOI) = ΔCT(GOI-condition A) − ΔCT(GOI condition B) with CD271⁺ and CD271⁻ for condition A and B, respectively.