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. 2019 May 22;10:2262. doi: 10.1038/s41467-019-10239-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Comparison of Cre expression levels in different tissues and mouse lines. a Western blot analysis of lung lysates from mice with the indicated genotypes, showing Cre (35kDa) and CreERT2 (70 kDa) expression differences in Tie2-Cre and Cdh5-CreERT2 mouse lines. b Western blot analysis of FAC-sorted endothelial cells (CD31-APC+) from the indicated mice, showing Cre (35kDa) and CreERT2 (70 kDa) expression in wild-type, MbTomato+ and MbTomato− cells. c Western blot analysis of FAC-sorted bone marrow-derived macrophages (BMDMs) obtained from the indicated mice. d, e Western blot analysis of lung and liver lysates from mice with the indicated genotypes, showing the increase in Cre protein levels in tissues of animals with expression of the Tg(iSuRe-Cre) allele. f Western blot analysis of heart lysates from mice with the indicated genotypes, showing no significant increase in Cre protein levels in tissues of MyHC-Cre + Tg(iSuRe-Cre) animals. g Western blot analysis of heart lysates showing that animals with recombination of the Gt(Rosa)26Sor-iSuRe-Cre allele in the germline, and with ubiquitous iSuRe-Cre expression in their hearts (not only MyHC-Cre + cardiomyocytes), have significantly lower Cre levels than MyHC-Cre + hearts. h–j Metabolic studies in adult littermate mice (9 months old, n = 4 per group) having or lacking ubiquitous expression of the Gt(Rosa)26Sor-iSuRe-Cre allele. Values in red below the blots indicate the fold change in Cre protein relative to Tg(iSuRe-Cre) allele expression. Error bars indicate StDev; NS nonsignificant. Two-tailed unpaired t-test. Source data are provided as a Source Data file