Figure 2.
Downregulation of MYH9 protein in Ang II-treated podocytes. (A) Lysates from cultured mouse podocytes were Western blotted with antibodies, specific to MYH9, MYH10 and MYH14 to determine their expression levels (n = 3). (B) Western blot demonstrating increased Ang II in diabetic db/db mice (n = 8) compared to nondiabetic db/m mice (n = 4). The blots were cropped from different parts of the same gel. (C) Real-time PCR and (D) Western blot demonstrating decreased MYH9 at the RNA and protein levels in Ang II (10−6 M)-stimulated podocytes in a time-dependent manner (n = 3). (E) Western blot of MYH9 in mouse podocytes stimulated by Ang II with or without treatment with losartan (Los, 10−6 M, n = 3 per group). Data are presented as the means ± SEM. Similar results were obtained in two independent experiments. *P < 0.05 versus MYH9, nondiabetic db/m mice and control podocytes. #P < 0.05 compared with podocytes treated with Ang II.