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. 2019 May 22;9:7715. doi: 10.1038/s41598-019-44156-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Autofluorescence (01, 04 and 07), fSHG collagen (02, 05 and 08) and bSHG collagen (03, 06 and 09). Images correspond to: invasive ductal carcinoma no special type (01, 02 and 03), classic invasive lobular (04, 05 and 06) and tubular (07, 08 and 09). Autofluorescence was included in this Figure to show tissue architecture. The images were log-transformed. For more examples see Supplementary Fig. 1. [bSHG, fSHG: backward and forward propagation second harmonic generation, respectively].