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. 2019 May 22;9:7735. doi: 10.1038/s41598-019-44154-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NMR spectral analysis of cultured Cordyceps. (a‒d) ¹H NMR spectra (a, −0.2 to 8.8 ppm; b, 0.9 to 1.1 ppm; c, 3.0 to 4.0 ppm; and d, 5.85 to 6.6) of the extracts of C. militaris cultured in a silkworm chrysalis medium (CMS) and in a rice medium (CMR). (c) Concentrations of the organic compounds in extracts from C. militaris cultured in a silkworm chrysalis medium and a rice medium determined by NMR spectroscopy. The extracts of natural C. militaris (n = 3) were compared with those of C. militaris cultured in a silkworm chrysalis medium and a rice medium (n = 5). The other abbreviations are the same as those in Fig. 1. Data are represented as the means ± SD.