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. 2019 May 22;10:2267. doi: 10.1038/s41467-019-09929-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Lamin A/C-mediated reduction of Na_v1.5 expression is confirmed in heart sections. a Immunostaining of paraffin-embedded heart sections from patients carrying the K219T mutation on Lamin A/C (bottom panels) and from two control subjects (top panels) targeting the Na_v1.5 sodium channel (red); nuclei were stained with DAPI (scale bars: 10 μm). b Histogram showing the quantification of the red fluorescent signal shown in a, corresponding to the Na_v1.5 protein, confirming reduced expression also in K219T-LMNA patient hearts. (a.u. is for arbitrary units). Data are relative to heart sections from three healthy controls (n = 11) and four mutant samples (n = 12). c PAT-ChIP analysis of heart sections from patients carrying the K219T mutation on LMNA (red bar) and from two control subjects (black bar), showing increased binding of Lamin A/C at the SCN5A promoter region in K219T-CMs. Chromatin was immunoprecipitated with an antibody against Lamin A/C and the resulting DNA was analysed by realtime PCR and presented as a percentage of input chromatin precipitated for the indicated region. All values are reported as means ± SD. *p < 0.05; ***p < 0.0001 (unpaired t-test)