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. 2019 May 22;9:7720. doi: 10.1038/s41598-019-44211-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HPAEC-PAC analysis of the product specificity of B. megaterium levansucrase variants. (A) FOS profile of variants R370H and K373R (from groups 2 and 3); (B) FOS produced by variants R370Q and R370Q/K373R/F419W (from group 4); and (C) FOS synthesized by variant K373H (from group 5). Products of the wild-type levansucrase (group 1) are shown as black dotted lines. Reactions were performed in Sörensen buffer using 2 U mL⁻¹ of each enzyme and 0.5 m sucrose. The products were analyzed after 24 h reaction, at comparable sucrose conversions for all variants (around 90–95%). This figure is composed of cropped chromatograms focused on the oligosaccharide products of variants; full-length chromatograms are included as Supplementary Information, Figs S5 and S6.