Table 1.
Primers of the PEDV genes
| Genes | Forward primer (5′–3′) | Reverse primer (5′–3′) | Position |
|---|---|---|---|
| PEDVORF1P1 | ATGGCTAGCAACCATGTCACATTGG | TGCATGCTTACCCTTACGTGGACC | 293–4285 |
| PEDV ORF1P2 | GCAAAGGCCATTGATGTTTATACCA | TCGAAGCACAAACCAGATGTACCAA | 4286–8033 |
| PEDV ORF1P3 | TCTTTATTAACTTCGACCAGTGGCA | GAACTTACGTGGTGGTTCTAGCTCA | 8034–11986 |
| PEDV ORF1P4 | ATTTCAGATAAACCGGACCTGCGTG | GCACAAAAACCATGCCAGGAACAAG | 11987–15954 |
| PEDV ORF1P5 | ATCGGTGAGTTTGTGTTTGAGAAGG | GTCAAAGCAGAACCACTTAGCATCC | 15955–18214 |
| PEDV ORF1P6 | ATGTGGTTCGCAAGCGTATAGTTCA | TCATTTGTTTACGTTGACCAAATGATTAGA | 18215–20633 |
| PEDV ORF3 | ATGTTTCTTGGACTTTTTCAATAC | TCATTCACTAATTGTAGCATACTCG | 24794–25448 |
| PEDVE | ATGCTACAATTAGTGAATGATAATGGG | TTATACGTCAATAACAGTACTGGGG | 25449–25686 |
| PEDVM | ATGTCTAACGGTTCTATTCCC | TTAGACTAAATGAAGCACTTTCTC | 25687–26396 |
| PEDVN | ATGGCTTCTGTCAGCTTTCAGGATCG | TTAATTTCCTGTGTCGAAGATCTCGTTGA | 26397–27704 |
| PEDV5UTR | ACTTAAAAAGATTTTCTATCTACGGATA | AGCCGGCAGTTACTGGTTT | 1–292 |
| PEDV3UTR | ACAATGTTAGACCGGCTTATCCT | GTGTATCCATATCAACACCGTCAG | 27705–28038 |
| PEDVS | ATGAAGTCTTTAACCTACTTCTGGTTGTT | TCACTGCACGTGGACCTTTTC | 20634–24793 |