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. 2019 May 22;9:7689. doi: 10.1038/s41598-019-44205-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative expression of (A) interlukin-10, (B) interleukin-8; (C) transforming growth factor β, (D) superoxide dismutase, (E) interferon-γ, (F) heat shock protein 90, (G) metalloproteinase 9, (H) glucocorticoid receptor 1 and (I) indoleamine 2,3- dioxygenase genes in the head-kidney of European seabass fed dietary treatments prior infection, and at 4, 24, 48 and 72 h after peritoneal infection with Phdp. Values are presented as means ± SD (n = 6). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post hoc test was used to identify differences in the experimental treatments. Different lowercase letters stand for significant differences among dietary treatments for the same time while different symbols stand for significant differences between times for the same diet.