Table 4.
Plasma lysozyme, peroxidase, alternative complement pathway (expressed as ACH50) and bactericidal activities as well as cortisol levels of European seabass fed dietary treatments during 2 and 4 weeks.
| Parameters | Dietary treatments | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| NTRP | CTRL | TRP 13 | TRP 17 | ||||||
| 2 weeks | 4 weeks | 2 weeks | 4 weeks | 2 weeks | 4 weeks | 2 weeks | 4 weeks | ||
| Lysozyme | (µg ml−1) | 8.09 ± 1.30 | 8.11 ± 3,90 | 9.58 ± 1.87 | 7.86 ± 2.23 | 9.07 ± 2.64 | 8.39 ± 1.94 | 7.42 ± 1.16 | 10.97 ± 3.36 |
| Peroxidase | (units ml−1) | 95.55 ± 9.21 | 118.63 ± 19.73 | 101.04 ± 113.19 | 123.71 ± 13.94 | 97.37 ± 16.96 | 135.49 ± 27.82 | 90.84 ± 18.05 | 139.23 ± 29.92 |
| ACH50 | (units ml−1) | 66.09 ± 14.55 | 92.16 ± 26.76 | 115.92 ± 60.05 | 66.74 ± 10.31 | 86.02 ± 21.51 | 86.84 ± 22.67 | 103.3 ± 35.62 | 119.60 ± 30.88 |
| Bactericidal activity | (%) | 12.64 ± 8.42 | 26.38 ± 11.86 | 24.24 ± 13,29 | 28.32 ± 7.64 | 17.23 ± 7.92 | 23.76 ± 14.90 | 16.24 ± 7.48 | 17.43 ± 11.36 |
| Cortisol | (ng ml−1) | 26.43 ± 10.12 | 25.99 ± 4.40b | 29.71 ± 16.07 | 27.74 ± 3.09b | 24.57 ± 13.49 | 37.00 ± 6.67a,b | 27.57 ± 8.73 | 43.60 ± 12.88a |
| Two-way ANOVA | |||||||||
| Parameters | Time | Diet | Time × Diet | ||||||
| Lysozyme | ns | ns | ns | ||||||
| Peroxidase | <0.001 | ns | ns | ||||||
| ACH50 | ns | ns | ns | ||||||
| Bactericidal activity | ns | ns | ns | ||||||
| Cortisol | 0.017 | ns | 0.044 | ||||||
Values are presented as means ± SD (n = 12). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post hoc test was used to identify differences in the experimental treatments. Different lowercase letters stand for significant differences among dietary treatments for the same time.