Table 6.
Haemoglobin, mean corpuscular haemoglobin (MCH), red blood cells (RBC) and white blood cells (WBC) in European seabass fed dietary treatments prior infection (0 h) and at 4, 24, 48 and 72 h after peritoneal injection with Phdp.
| Parameters | Dietary treatments | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| NTRP | CTRL | ||||||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | 0 h | 4 h | 24 h | 48 h | 72 h | ||
| Haemoglobin | (g dl) | 0.87 ± 0.33 | 0.96 ± 0.26 | 1.23 ± 0.20 | 1.86 ± 0.37 | 1.68 ± 0.17 | 0.70 ± 0.25 | 0.77 ± 0.13 | 1.33 ± 0.32 | 2.12 ± 0.34 | 1.85 ± 0.31 |
| MCH | (pg cell−1) | 4.30 ± 1.40 | 6.24 ± 0.13 | 6.08 ± 1.32 | 9.82 ± 0.88 | 7.53 ± 3.52 | 3.48 ± 0.66 | 3.21 ± 0.83 | 6.95 ± 2.52 | 10.31 ± 2.16 | 18.52 ± 2.00 |
| RBC | (×106 µl−1) | 2.03 ± 0.37 | 1.86 ± 0.74 | 2.06 ± 0.23 | 1.89 ± 0.32 | 1.81 ± 0.34 | 1.96 ± 0.48 | 2.45 ± 0.27 | 1.99 ± 0.23 | 2.11 ± 0.46 | 2.24 ± 0.40 |
| WBC | (×104 µl−1) | 9.14 ± 3.57 | 5.12 ± 1.89 | 7.43 ± 1.45 | 9.38 ± 2.45 | 8.68 ± 2.49 | 9.36 ± 2.32 | 6.00 ± 1.44 | 6.92 ± 2.07 | 11.70 ± 3.30 | 11.47 ± 4.00 |
| Parameters | Dietary treatments | ||||||||||
| TRP 13 | TRP 17 | ||||||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | 0 h | 4 h | 24 h | 48 h | 72 h | ||
| Haemoglobin | (g dl) | 0.72 ± 0.27 | 0.95 ± 0.23 | 1.12 ± 0.15 | 2.05 ± 0.40 | 1.94 ± 0.57 | 0.80 ± 0.10 | 0.80 ± 0.18 | 1.14 ± 0.27 | 2.15 ± 0.65 | 1.90 ± 0.57 |
| MCH | (pg cell−1) | 3.39 ± 1.00 | 4.10 ± 0.73 | 5.45 ± 0.63 | 10.45 ± 2.56 | 10.42 ± 2.64 | 3.62 ± 0.62 | 3.65 ± 0.72 | 5.76 ± 1.33 | 10.88 ± 3.90 | 10.49 ± 3.30 |
| RBC | (×106 µl−1) | 2.14 ± 0.45 | 2.36 ± 0.58 | 2.08 ± 0.29 | 2.03 ± 0.45 | 1.87 ± 0.26 | 2.23 ± 0.24 | 2.20 ± 0.33 | 2.00 ± 0.31 | 2.07 ± 0.39 | 1.84 ± 0.22 |
| WBC | (×104 µl−1) | 8.33 ± 2.62 | 6.05 ± 1.64 | 6.85 ± 1.30 | 11.43 ± 7.65 | 8.85 ± 2.25 | 10.10 ± 1.82 | 5.42 ± 1.44 | 7.10 ± 1.69 | 9.53 ± 3.51 | 8.92 ± 1.22 |
| Two-way ANOVA | |||||||||||
| Parameters | Time | Diet | Time × Diet | Time | |||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | |||||||
| Haemoglobin | <0.001 | ns | ns | C | C | B | A | A | |||
| MCH | <0.001 | ns | ns | C | BC | B | A | A | |||
| RBC | ns | ns | ns | — | — | — | — | — | |||
| WBC | <0.001 | ns | ns | AB | C | B | A | AB | |||
Values are presented as means ± SD (n = 9 for 0 h and n = 6 for the remaining times). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post hoc test was used to identify differences in the experimental treatments. Different capital letters indicate differences in time regardless diets.