Table 7.
Absolute values of peripheral blood leucocytes (thrombocytes, lymphocytes, monocytes and neutrophils) of European seabass fed dietary treatments prior infection (0 h) and at 4, 24, 48 and 72 h after peritoneal injection with Phdp.
| Parameters | Dietary treatments | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NTRP | CTRL | ||||||||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | 0 h | 4 h | 24 h | 48 h | 72 h | ||||
| Thrombocytes | (×104 µl−1) | 4.87 ± 1.53 | 3.60 ± 1.34 | 5.17 ± 1.02 | 5.35 ± 1.09 | 4.86 ± 1.29 | 5.62 ± 1.38 | 3.97 ± 0.85 | 4.89 ± 1.67 | 5.87 ± 1.30 | 6.34 ± 2.20 | ||
| Lymphocytes | 3.97 ± 2.18 | 1.22 ± 0.43 | 1.65 ± 0.25 | 3.13 ± 1.54 | 2.78 ± 1.25 | 3.3 ± 1.22 | 1.91 ± 0.79 | 1.99 ± 0.80 | 4.45 ± 1.92 | 3.83 ± 1.70 | |||
| Monocytes | 0.16 ± 0.07£ | 0.16 ± 0.13£ | 0.11 ± 0.09b,£ | 0.29 ± 0.09*,£ | 0.69 ± 0.31* | 0.17 ± 0.15£ | 0.10 ± 0.09£ | 0.69 ± 0.27a,* | 0.30 ± 0.15*,£ | 0.78 ± 0.46* | |||
| Neutrophils | 0.14 ± 0.08 | 0.24 ± 0.25 | 0.51 ± 0.30b | 0.61 ± 0.17 | 0.36 ± 0.30 | 0.18 ± 0.12£ | 0.19 ± 0.07£ | 0.74 ± 0.27a,b,*,£ | 1.07 ± 0.44* | 0.52 ± 0.17*,£ | |||
| Parameters | Dietary treatments | ||||||||||||
| TRP 13 | TRP 17 | ||||||||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | 0 h | 4 h | 24 h | 48 h | 72 h | ||||
| Thrombocytes | (×104 µl−1) | 4.85 ± 1.30 | 4.10 ± 1.05 | 4.69 ± 0.91 | 6.03 ± 3.26 | 5.37 ± 1.27 | 6.07 ± 0.84 | 3.73 ± 1.12 | 2.91 ± 2.14 | 4.63 ± 1.30 | 5.59 ± 1.35 | ||
| Lymphocytes | 3.18 ± 1.48 | 1.27 ± 0.61 | 1.19 ± 0.30 | 2.82 ± 1.41 | 1.88 ± 0.56 | 3.82 ± 1.21 | 1.15 ± 0.28 | 0.55 ± 0.50 | 2.72 ± 1.68 | 2.14 ± 0.73 | |||
| Monocytes | 0.22 ± 0.14£ | 0.10 ± 0.07£ | 0.08 ± 0.07b,£ | 0.34 ± 0.21* | 0.55 ± 0.29* | 0.15 ± 0.11£ | 0.11 ± 0.05£ | 0.06 ± 0.02b,£ | 0.30 ± 0.18* | 0.37 ± 0.28* | |||
| Neutrophils | 0.08 ± 0.04§ | 0.39 ± 0.10*,£,§ | 0.88 ± 0.31a,b,* | 0.74 ± 0.51*,£ | 0.21 ± 0.07£,§ | 0.10 ± 0.06§ | 0.38 ± 0.20£,§ | 1.23 ± 0.26a,* | 0.74 ± 0.55*£ | 0.25 ± 0.20£,§ | |||
| Two-way ANOVA | |||||||||||||
| Parameters | Time | Diet | Time × Diet | Time | Diet | ||||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | NTRP | CTRL | TRP 13 | TRP 17 | |||||
| Thrombocytes | <0.001 | ns | ns | A | B | AB | A | A | — | — | — | — | |
| Lymphocytes | <0.001 | 0.005 | ns | A | B | B | A | A | AB | A | B | B | |
| Monocytes | <0.001 | ns | 0.001 | B | B | B | B | A | — | — | — | — | |
| Neutrophils | <0.001 | ns | 0.004 | C | BC | A | A | B | — | — | — | — | |
Values are presented as means ± SD (n = 9 for time 0 h and n = 6 for the remaining times). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post hoc test was used to identify differences in the experimental treatments. Different lowercase letters stand for significant differences among dietary treatments for the same time while different symbols stand for significant differences between times for the same diet. Different capital letters indicate differences in time regardless diets or among diets regardless time.