Table 8.
Absolute values of peritoneal total leucocytes, as well as lymphocytes, macrophages and neutrophils of European seabass fed dietary treatments at 4, 24, 48 and 72 h after peritoneal injection with Phdp.
| Parameters | Dietary treatments | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| NTRP | CTRL | ||||||||
| 4 h | 24 h | 48 h | 72 h | 4 h | 24 h | 48 h | 72 h | ||
| Leucocytes | (×104 µl−1) | 9.89 ± 4.85 | 8.95 ± 4.95 | 7.83 ± 2.56 | 8.26 ± 2.91 | 11.51 ± 3.36 | 10.33 ± 4.60 | 13.63 ± 6.20 | 13.83 ± 8.04 |
| Lymphocytes | 3.78 ± 1.44 | 3.31 ± 2.16 | 3.19 ± 1.26 | 2.93 ± 1.62 | 2.96 ± 122 | 3.16 ± 1.68 | 5.17 ± 2.84 | 4.45 ± 2.13 | |
| Macrophages | 3.23 ± 1.88 | 3.48 ± 1.72 | 2.72 ± 1.06 | 3.40 ± 1.10 | 5.24 ± 1.45 | 4.68 ± 2.48 | 5.29 ± 2.27 | 5.49 ± 4.00 | |
| Neutrophils | 2.88 ± 1.65 | 2.16 ± 1.16 | 1.91 ± 0.60 | 1.98 ± 1.17 | 1.89 ± 0.78 | 2.3 ± 1.04 | 3.18 ± 1.32 | 4.13 ± 2.95 | |
| Parameters | Dietary treatments | ||||||||
| TRP 13 | TRP 17 | ||||||||
| 4 h | 24 h | 48 h | 72 h | 4 h | 24 h | 48 h | 72 h | ||
| Leucocytes | (×104 µl−1) | 8.71 ± 4.97 | 8.90 ± 2.77 | 9.88 ± 4.70 | 8.98 ± 7.27 | 10.95 ± 2.37 | 10.70 ± 4.42 | 9.35 ± 3.37 | 10.36 ± 4.71 |
| Lymphocytes | 3.13 ± 1.24 | 2.27 ± 0.68 | 3.58 ± 2.19 | 3.55 ± 3.06 | 4.73 ± 1.21 | 4.01 ± 1.46 | 3.55 ± 1.42 | 3.64 ± 1.53 | |
| Macrophages | 3.49 ± 1.67 | 4.12 ± 1.48 | 4.38 ± 1.58 | 2.28 ± 2.28 | 3.53 ± 0.95 | 3.85 ± 2.34 | 3.76 ± 1.53 | 3.27 ± 1.64 | |
| Neutrophils | 3.44 ± 1.57 | 2.41 ± 0.90 | 3.00 ± 1.17 | 2.92 ± 1.06 | 2.50 ± 0.55 | 3.09 ± 1.49 | 2.08 ± 0.77 | 3.49 ± 2.13 | |
| Two-way ANOVA | |||||||||
| Parameters | Time | Diet | Time × Diet | Diet | |||||
| NTRP | CTRL | TRP 13 | TRP 17 | ||||||
| Leucocytes | ns | 0.025 | ns | B | A | AB | AB | ||
| Lymphocytes | ns | ns | ns | — | — | — | — | ||
| Macrophages | ns | 0.002 | ns | B | A | B | B | ||
| Neutrophils | ns | ns | ns | — | — | — | — | ||
Values are presented as means ± SD (n = 6). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post hoc test was used to identify differences in the experimental treatments. Different capital letters indicate differences among diets regardless time.