Table 9.
Plasma lysozyme, peroxidase, alternative complement pathway (expressed as ACH50) and bactericidal activities as well as cortisol of European seabass fed dietary treatments prior infection (0 h) and at 4, 24, 48 and 72 h after peritoneal injection with Phdp.
| Parameters | Dietary treatments | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NTRP | CTRL | ||||||||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | 0 h | 4 h | 24 h | 48 h | 72 h | ||||
| Lysozyme | (µg ml−1) | 8.11 ± 3.90 | 7.42 ± 1.2 | 5.00 ± 1.71 | 4.41 ± 2,47 | 8.11 ± 5.56 | 7.86 ± 2.23 | 9.26 ± 2.42 | 5.11 ± 2.45 | 5.95 ± 2.90 | 7.86 ± 3.11 | ||
| Peroxidase | (units ml−1) | 118.63 ± 19.73 | 93.86 ± 19.00 | 115.84 ± 32.05 | 128.53 ± 45.05 | 126.55 ± 27.64 | 123.71 ± 13.94 | 96.83 ± 13.82 | 118.89 ± 28.75 | 111.48 ± 29.16 | 113.83 ± 31.17 | ||
| ACH50 | (units ml−1) | 92.16 ± 26.76 | 120.85 ± 26.09 | 85.32 ± 34.77 | 94.64 ± 23.34 | 102.78 ± 17.87 | 66.74 ± 10.31 | 142.35 ± 24.57 | 96.15 ± 28.66 | 103.52 ± 26.17 | 100.60 ± 15.14 | ||
| Bactericidal activity | (%) | 26.38 ± 11.86 | 30.08 ± 2.81 | 38.97 ± 7.58 | 27.06 ± 13.48 | 28.70 ± 8.20 | 28.32 ± 7.64 | 28.49 ± 8.96 | 43.81 ± 9.44 | 29.84 ± 4.39 | 32.04 ± 4.48 | ||
| Cortisol | (ng ml−1) | 25.99 ± 4.40£ | 50.44 ± 12.37£,* | 65.43 ± 8.23£,* | 103.11 ± 68.18a,* | 72.22 ± 22.52£,* | 27.74 ± 3.09 | 59.24 ± 5.00 | 51.05 ± 14.29 | 45.76 ± 8.90b | 53.13 ± 23.37 | ||
| Parameters | Dietary treatments | ||||||||||||
| TRP 13 | TRP 17 | ||||||||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | 0 h | 4 h | 24 h | 48 h | 72 h | ||||
| Lysozyme | (µg ml−1) | 8.39 ± 1.94 | 7.84 ± 3.66 | 6.23 ± 1.75 | 6.17 ± 41.7 | 8.96 ± 2.73 | 10.97 ± 3.36 | 11.44 ± 5.23 | 7.61 ± ± 1.84 | 5.50 ± 2.47 | 10.32 ± 3.4 | ||
| Peroxidase | (units ml−1) | 135.49 ± 27.82 | 108.82 ± 25.98 | 115.62 ± 16.20 | 102.16 ± 32.27 | 87.33 ± 14.55 | 139.23 ± 29.92 | 103.03 ± 21.26 | 113.44 ± 10.43 | 114.16 ± 31.95 | 85.29 ± 28.00 | ||
| ACH50 | (units ml−1) | 86.84 ± 22.67 | 58.42 ± 0.00 | 92.74 ± 28.44 | 139.49 ± 22.53 | 89.57 ± 24.25 | 119.60 ± 30.88 | 81.12 ± 12.14 | 99.24 ± 23.844 | 108.52 ± 30.32 | 126.56 ± 31.02 | ||
| Bactericidal activity | (%) | 23.76 ± 14.90 | 33.62 ± 14.35 | 35.67 ± 4.30 | 30.14 ± 6.65 | 34.74 ± 12.37 | 17.43 ± 11.36 | 34.40 ± 9.59 | 43.17 ± 15.5 | 28.92 ± 5.75 | 31.26 ± 12.13 | ||
| Cortisol | (ng ml−1) | 37.00 ± 6.67 | 50.54 ± 11.27 | 44.43 ± 8.51 | 61.70 ± 10.89a,b | 32.62 ± 11.10 | 43.60 ± 12.88 | 42.00 ± 6.53 | 66.16 ± 17.87 | 33.44 ± 9.15b | 44.57 ± 17.03 | ||
| Two-way ANOVA | |||||||||||||
| Parameters | Time | Diet | Time × Diet | Time | Diet | ||||||||
| 0 h | 4 h | 24 h | 48 h | 72 h | NTRP | CTRL | TRP 13 | TRP 17 | |||||
| Lysozyme | <0.001 | 0.039 | ns | A | AB | B | B | A | B | AB | AB | A | |
| Peroxidase | 0.006 | ns | ns | A | B | AB | AB | A | — | — | — | — | |
| ACH50 | ns | ns | ns | — | — | — | — | — | — | — | — | — | |
| Bactericidal activity | <0.001 | ns | ns | B | AB | A | B | AB | — | — | — | — | |
| Cortisol | <0.001 | 0.007 | 0.001 | B | AB | A | A | AB | A | AB | B | AB | |
Values are presented as means ± SD (n = 9 for time 0 h and n = 6 for the remaining times). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post hoc test was used to identify differences in the experimental treatments. Different lowercase letters stand for significant differences among dietary treatments for the same time while different symbols stand for significant differences between times for the same diet. Different capital letters indicate differences in time regardless diets or among diets regardless time.