Table 1.

Forward and reverse primers for T-cell transcription factors

Target	Forward primer	Reverse primer
T-bet	5′-CAACGCTTCCAACACGCAT-3′	5′-GACTCAAAGTTCTCCCGGAA-3′
GATA3	5′-TCATTAAGCCCAAGCGAAGG-3′	5′-GTCCCCATTGGCATTCCTC-3′
RORC	5′-GCAGCGCTCCAACATCTTCT-3′	5′-ACGTACTGAATGGCCTCGGT-3′
FOXP3	5′-CACCTGGCTGGGAAAATGG-3′	5′-GGAGCCCTTGTCGGATGA-3′
β-actin	5′-CGGGAAATCGTGCGTGAC-3′	5′-GGAAGGAAGGCTGGAAGAG-3′

See section “Quantification of mRNA Expression by Real-Time PCR” for explanation of targets