Table 1.

Characterization of the DNA markers used in this study

Chromosome arm	Gene/locus		Primer sequence (5′–3′)	T m	Product size (bp)
1HL	HvCENH3αa	F	AGA AGA AGA TCG GGT CCG CTA	66.4	801
		R	GTG CAA ACG GGA TGA GAA AAT T	66.2
2HS	HvM36b	F	TCC AGC CGA CAA TTT CTT G	64.2	114
		R	AGT ACT CCG ACA CCA CGT CC	64.5
3HL	ABG377a	F	GCT GCT ATG AGG AGA GAA CC	60.6	507
		R	TGG TAT GAA ACA GGT GAA TA	55.4
4HL	ABG498a	F	TTA CTG AAG AAA AAC CTG TC	53.4	509
		R	CTG ACT ACT GGA TGG ACC AC	59.6
5HL	HvCsIF7a	F	CCC TGC TCT TGC TTG TCG TAG	66.1	121
		R	TAG CCA AGC AAT TGC ATT T	60.8
6HS	HvCENH3βa	F	ATG GCT CGC ACG AAG AAA ACG G	73.1	453
		R	GTC GGC TTG CTC TCC TTC TTG TTC G	73.4
7HS	ABC465a	F	CAC GAC AGA CGG ACC AAA TG	66.6	438
		R	GCT ACT GGG ACA AAA TCT CC	60.1
Control (UPP)	Wheat cDNA (TA30797)	F	GCCGTGTCCATGCCAGTG	58.1	195
		R	TTAGCCTGAACCACCAGTGC	60.1

UPP universal plant primers (Paolacci et al. (2009), F forward, R reverse

^aSTS, sources: HvCENH3α and β, Sanei et al. (2011); ABG377/498 and ABC465, Kleinhofs et al. (1993); HvCsIF7, Burton et al. (2008)

^b(GA)₁₃ SSR, source: Liu et al. (1996)