FIG 9.
Overexpression of TtpA protects against carbenicillin treatment; protection requires EipB. (A) Growth on SBA plates containing 3 μg/ml of carbenicillin with or without 2 mM IPTG of serially diluted (10-fold dilution) B. ovis wild-type (WT) and ΔeipB strains expressing wild-type EipB (Plac-eipB) or TtpA (Plac-ttpA). B. ovis strains carrying the pSRK empty vector were used as a control. The days of growth at 37°C and 5% CO2 are reported for each plate. A representative picture of the different plates is presented. (B) Enumerated CFU after growth on SBA plates containing 3 μg/ml of carbenicillin with or without 2 mM IPTG of serially diluted (10-fold dilution) B. ovis wild-type and ΔeipB strains ectopically expressing eipB or ttpA. EV strains and SBA plates with no carbenicillin and with or without IPTG were used as controls. This experiment was independently performed twice with two different clones each time, and all plate assays were done in triplicate. Each data point indicates the mean ± the standard error of the mean.