TABLE 1.
Plasmids used in cloning reported in this study
| Plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| pET15b | E. coli cloning vector, Ampra | Novagen |
| pET-hspR | HspR residues 1–126 cloned in pET15b | This study |
| pET-hrcA | HrcA residues 1–343 cloned in pET15b | This study |
| pET15b-hspRΔ10 | HspR residues 1–116 cloned in pET15b | This study |
| pET15b-hrcAΔ18 | HrcA residues 1–325 cloned in pET15b | This study |
| pGEX-4T-1 | E. coli cloning vector, Ampr | GE Healthcare |
| pGEX-hrcA | HrcA residues 1–343 cloned in pGEXT-4T-1 | This study |
| pGEX-hspR | HspR residues 1–126 cloned in pGEXT-4T-1 | 11 |
| pGEX-phoP | PhoP residues 1–247 cloned in pGEX-4T-1 | 32 |
| p19Kpro | Mycobacterial expression vector, Hygrb | 14 |
| p19Kpro-phoP | PhoP residues 1–247 cloned in p19Kpro | 15 |
| p19Kpro-hrcA | HrcA residues 1–343 cloned in p19Kpro | This study |
| pSTKi | Integrative mycobacterial expression vector, Kanrc | 13 |
| pSTKi-hrcA | HrcA residues 1–343 cloned in pSTKi | This study |
| pSTKi-hspR | HspR residues 1–126 cloned in pSTKi | This study |
| pSTKi-hrcAΔ18 | HrcA residues 1–325 cloned in pSTKi | This study |
| pSTKi-hspRΔ10 | HspR residues 1–116 cloned in pSTKi | This study |
| pUAB300b | Episomal mycobacteria-E. coli shuttle plasmid | 16 |
| pUAB300-hrcA | HrcA residues 1–343 cloned in pUAB300 | This study |
| pUAB300-hspRΔ10 | HspR residues 1–116 cloned in pUAB300 | This study |
| pUAB300-hrcAΔ18 | HrcA residues 1–325 cloned in pUAB300 | This study |
| pUAB400c | Integrative mycobacterium-E. coli shuttle plasmid | 16 |
| pUAB400-phoP | PhoP residues 1–247 cloned in pUAB400 | 11 |
| pUAB400-phoPN | PhoP residues 1–141 cloned in pUAB400 | This study |
| pUAB400-phoPC | PhoP residues 141–247 cloned in pUAB400 | This study |
a
Ampr, ampicillin resistant.
b
Hygr, hygromycin resistant.
c
Kanr, kanamycin resistant.