FIG 2.

Analysis of the effects of ethanol on treZ and osmC transcript levels and intracellular trehalose in P. aeruginosa wild-type and ΔalgU strains. (A and B) treZ (A) and osmC (B) transcript levels relative to rpoD after growth in the absence and presence of 1% ethanol in buffered LB for 16 h. a-b, P < 0.0001 (two-way ANOVA and Tukey’s multiple-comparison test). (C and D) Trehalose levels in wild type, ΔalgU, and ΔalgU plus algU strains at the native locus in buffered LB (C) and M63 medium (D) with and without 1% ethanol for 16 h. (C) a-b, P < 0.002; a-c, P < 0.0009; b-c, not significant (NS). (D) a-b, P < 0.0001. (P values based on two-way ANOVA and Tukey’s multiple-comparison test.) The data are representative of the results of at least three independent experiments, each with at least three biological replicates. The error bars indicate standard deviations.