Skip to main content
. 2019 May 22;201(12):e00794-18. doi: 10.1128/JB.00794-18

FIG 5.

FIG 5

Trehalose accumulation in response to NaCl requires TreYZ and AlgU. (A) NaCl (500 mM) stimulates trehalose accumulation. (B) NaCl-stimulated accumulation of trehalose is dependent on the TreYZ pathway. (C) NaCl-stimulated trehalose accumulation requires algU, and dksA and spoT contribute. (D) NaCl-stimulated trehalose accumulation is independent of relA. (E) Trehalose accumulation in NaCl in the ΔlasR ΔrhlR strain is similar to that in the wild type. Cultures were grown for 16 h in M63 medium with 1% ethanol or 500 mM NaCl as indicated. The data are representative of the results of two or more experiments, each with at least 3 biological replicates. The statistics are based on one-way ANOVA (A) or two-way ANOVA (B, C, and D) and Tukey’s multiple-comparison test. (A) a-b, P = 0.0005; a-c and b-c, P < 0.0001. (B) a-b, P < 0.0001. (C) a-b, a-c, a-d, and b-c, P < 0.0001. (D) a-b, a-c, a-d, and b-c, P < 0.0001. (E). a-b and a-c, P < 0.0001; b-c, P = 0.0007. The error bars indicate standard deviations.