Figure 9.

LA-ICP-MS imaging in human Wilson’s disease. LA-ICP-MS imaging for copper (Cu), iron (Fe), manganese (Mn), and zinc (Zn) was done in two serial cuts from a Wilson’s disease patient. Carbon (C) in each section was measured in parallel and used for normalization. Light microscopic (LM) images of the respective cryosections taken at a fourfold magnification are depicted for orientation. Concentrations of all trace elements are given in µg/g liver tissue, except C given in %. Please note the high reproducibility of the LA-ICP-MS imaging measurements in the serial cuts. The permission to image human liver samples is covered by an ethical vote (EK 186/15) from the relevant authority (Institutional Ethics Review Board of the Medical Faculty at the RWTH University Hospital Aachen). More details about patient’s characteristics and experimental setup for LA-ICP-MS imaging human liver samples are published elsewhere (48).