Fig. 3.

1020-site optical recordings of neural responses to N.IX (A) and N.X (B) stimulation in an E14 mouse brainstem en bloc preparation. The recordings were made with a magnification of x10 to detect optical responses from a wide region of the brainstem. Either N.IX or N.X stimulation elicited neural responses in three areas (Areas 1–3) on the stimulated side. Area 1 (pink shadow) was located at the caudal level of the N.IX/N.X root, and corresponded to the NTS. Areas 2 and 3 (orange and green shadows, respectively) were discerned in the rostral medulla and pons, respectively. On the contralateral side, small slow signals were detected in several positions as typified by arrowheads. The colored-response areas were determined by eye, whereas the precise maps are shown in Fig. 5B. G.VIII, vestibulo-cochlear ganglion.