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. 2019 Apr 22;116(10):2009–2022. doi: 10.1016/j.bpj.2019.04.012

Figure 3.

Figure 3

Contribution of ADP to the mechanically stimulated purinergic signal. (A) Representative [Ca2+]i elevations in Fura2-loaded C2-OBs stimulated by 1 μM ATP, 1 μM ADP, 10 μM AMP, or 10 μM adenosine (Ado) are shown. (B and C) 1 μM ATP was added to cultures of C2-OB osteoblasts, RAW 264.7 osteoclast precursors, and K562 erythrocyte-like cells; ATP degradation was measured (B); and decay time constants τdecay were estimated (C), data are means ± SEM, n = 3 per time point per cell type. (D) Degradation of 1 μM ATP by C2-OBs in the presence of the ectonucleotidase inhibitor ARL 67156 (10 μM) or the alkaline-phosphatase inhibitor orthovanadate (10 μM) is shown, data are means ± SEM, the sample sizes are shown in parentheses. For (B)–(D), solid curves show fitted exponential functions (Table S1). To see this figure in color, go online.