Table 1.
Transposon-disrupted genes identified from SBW25 ΔrccA cells displaying a wild-type-like swarm motility.
| Gene hit | Distance from 5′ | Promoter direction | Gene function |
|---|---|---|---|
| PFLU0179 | 36 aa | Plus | Putative phage integrase |
| PFLU0179 | N/D | N/D | Putative phage integrase |
| PFLU0920 | 130 aa | Plus | Glycerate dehydrogenase |
| PFLU1255 | 612 aa | Plus | Glycerol-3-phosphate acyltransferase |
| PFLU1268 | 749 aa | Minus | PII-uridylyl-transferase (glnD) |
| PFLU1513 | N/D | Minus | Putative glutamine synthetase |
| PFLU4766 | 138 aa | Minus | Acetyl-CoA synthetase |
| PFLU4766 | 399 aa | Plus | Acetyl-CoA synthetase |
| PFLU0087 | 1 aa | Plus | Two-component system sensor kinase (kinB) |
| PFLU5329 | 183 aa | Minus | Putative sensory box GGDEF/EAL domain protein |
| PFLU0156 | 40 aa | Minus | Hypothetical protein |
| PFLU0426 | 354 aa | Minus | Conserved hypothetical protein |
N/D, not determined. Promoter direction refers to the direction of the Tet promoter in relation to the disrupted open reading frame. Gene function refers to annotated functions according to www.pseudomonas.com (Winsor et al., 2011).