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. 2019 Apr 9;116(10):1941–1951. doi: 10.1016/j.bpj.2019.04.001

Figure 5.

Figure 5

Expression and spectroscopic investigation of KR2-WT and KR2-R109Q in E. coli. (A) Light-induced pH changes in suspensions of E. coli cells expressing KR2-WT or KR2-R109Q (in 100 mM NaCl) are shown. (B) Absorption spectra of purified KR2 (upper panel) and KR2-R109Q (lower panel) recorded at pH 8.0 (left) and pH 10.0 (right) and variable ionic conditions are shown: 150 mM NaCl (red), 150 mM KCl (green), or 150 mM LiCl (yellow). (C and D) Reconstructed absorption difference surface plots are shown from flash photolysis measurements on purified recombinant (C) KR2 (150 mM NaCl) and (D) KR2-R109Q (150 mM NaCl (pH 8) and 150 mM KCl (pH 10)). The spectral data were globally fitted, and the resulting photointermediate decay τ values are indicated in the surface plots. To see this figure in color, go online.