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. 2019 Mar 21;24:64–79. doi: 10.1016/j.molmet.2019.03.008

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

FTH is essential to sustain mitochondrial function in fat. Mean relative mRNA expression ± SD in (A) WAT and (B) BAT from Fth^fl/fl (n = 5) and Fth^R26Δ/Δ mice (n = 7) mice, 7 days after tamoxifen treatment. Data are represented as mean ± SD, pooled from 2 independent experiments. (C) Representative electron micrograph of inguinal WAT from Fth^R26Δ/Δ and Fth^fl/fl mice on day 7 post tamoxifen administration. (D) Relative number of mitochondria in inguinal WAT from Fth^fl/fl (n = 8) and Fth^R26Δ/Δ (n = 11) mice on day 7 after tamoxifen administration. Data are represented as mean ± SD, pooled from 3 independent experiments. (E) Relative expression of ETC complex I (i.e. Nadh dehydrogenase), II (i.e. succinate dehydrogenase), III (i.e. subunit Core 2/Uqcrc2), IV (subunit I/Cox1), V (i.e. ATP synthase) proteins and FTH, detected by western blot in WAT from Fth^fl/fl and Fth^R26Δ/Δ mice (n = 3 per genotype), 7 days after tamoxifen administration. Histone H3 was used as loading control. (F) Quantification of different ETC complex proteins detected in (E). Data are represented as mean ± SD. (G) Representative electron micrograph of the BAT from Fth^R26Δ/Δ and Fth^fl/fl mice on day 7 after tamoxifen administration. (H) Relative number of mitochondria in BAT from Fth^fl/fl (n = 10) and Fth^R26Δ/Δ (n = 11) mice. Data represented as mean ± SD, pooled from 3 independent experiments. (I) Relative expression of ETC complex I (i.e. Nadh dehydrogenase), II (i.e. succinate dehydrogenase), III (i.e. subunit Core 2/Uqcrc2), IV (subunit I/Cox1), V (i.e. ATP synthase) proteins, and FTH detected by western blot in the BAT of Fth^fl/fl and Fth^R26Δ/Δ mice (n = 3 per genotype), 7 days after tamoxifen administration. Histone H3 was used as loading control. (J) Quantification of different ETC complex (C) proteins, detected in (I). Data are represented as mean ± SD. Mann–Whitney test was used for comparison between two groups. NS: non-significant. *P < 0.05, **P < 0.01, ***P < 0.001. Arrows in (C) and (G) highlight mitochondria and * highlights fat reservoir.