Body weight, thermogenesis, and adipogenesis in young Lcn2 Tg mice on a RCD and exposed to an ambient temperature of 22°C. Body weight (A) and tissue weight (B) of WT and Lcn2 Tg male mice on a RCD at 16 weeks of age, n = 8–10 mice/group. Expression of thermogenic and mitochondrial genes in iWAT (C) and BAT (D) from 16-week-old WT and Lcn2 Tg male mice. UCP1 protein expression (E) in BAT and iWAT and UCP1 staining by immunohistochemistry (F) in iWAT of WT and Lcn2 Tg mice at 16 weeks of age. Adipogenic gene expression in BAT (G), iWAT (H), and epididymal WAT (eWAT) (I) from 16-week-old WT and Lcn2 Tg male mice (n = 8–10 mice/group).