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. 2019 Mar 20;24:18–29. doi: 10.1016/j.molmet.2019.03.007

Figure 5.

Figure 5

AMPK phosphorylation and adipogenic markers in adipose tissue from middle-aged and old mice. (A–D) AMPKThr172 phosphorylation levels and quantification in BAT, eWAT, and iWAT from 4-month- and 10-momth-old WT and Lcn2 Tg male mice (n = 6–8 mice/group). (E and F) AMPK Thr172 phosphorylation levels and quantification (fold change) in BAT, eWAT, and iWAT from 18-month-old WT and Lcn2 Tg male mice (n = 5–7 mice/group). (G) Adipogenic gene expression in iWAT from 4-month- and 10-month-old WT male mice (n = 6–8 mice/group). (H) Protein levels of PPARγ and adiponectin in iWAT from 10-month-old WT and Lcn2 Tg male mice. (I and J) Adiponectin protein expression and quantification in BAT, iWAT, and eWAT from 18-month-old WT and Lcn2 Tg male mice (n = 5–7 mice/group. (K) Glut 4 gene expression in iWAT and eWAT from 4-month- and 10-month-old WT and Lcn2 Tg male mice (n = 6–8 mice/group). (L) Serum levels of leptin at 4 months (young), 10 months (middle aged), and 18 months (old) of age from WT and Lcn2 Tg male mice. *p < 0.05 vs WT of same age; **p < 0.01 vs WT of same age; #p < 0.05, ##p < 0.01, ###p < 0.001 vs 4 m (young).