FIG 5.

Experimental challenge of surviving goats following infection with the 9.2.3B obg subclone. Shown are survival rates and clinical parameters measured during in vivo challenge with wild-type M. mycoides subsp. capri GM12 (10⁹ CFU per animal) of the 7 animals that survived the initial infection with M. mycoides subsp. capri obg 9.2.3B. Due to the small number of animals and the heterogeneity of data, individual curves for clinical parameters are shown in panels B to F. (A) Six out of the seven animals were euthanized due to severity of disease between days 4 and 10 postchallenge. (B) All but one of the seven animals had fever (defined as a rectal temperature of >39.5°C), with peak temperatures ranging from 40.3°C to 41.8°C. One animal did not show an elevated temperature (animal CM118). (C) The white blood cell count dropped initially in 5 of the 7 animals but remained within the normal range throughout the study. (D) Weight loss was seen during severe disease in 5 animals. Goat CM118 lost weight after the other animals in the group had been euthanized but started to recover at around 20 days postchallenge. (E) Breathing frequency was within the normal range throughout the challenge experiment. (F) At the peak of severity of disease, the individual heart rates of animals CM193, CM051, CM129, and CM126 increased. The average heart rate remained within the normal range throughout the trial.