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. 2019 May 22;4(3):e00030-19. doi: 10.1128/mSphere.00030-19

TABLE 1.

M. mycoides subsp. capri obg mutants and subclones obtained in this studyc

M. mycoides
subsp. capri
obg mutant		 obg
nucleotide
change(s)
(position)a 		Obg
amino acid
change(s)a 		NadE
amino acid
change		 TS
phenotype
of mutantb 		M. mycoides
subsp. capri
obg subclone		 TS
phenotype
of cloneb
11.1 GAT>AAC
(253/255)		 Asp85Asn None ND ND
15.2 GGG>CGT
(369/371)		 Gly124Arg None ND ND
69.3 GGG>AGA
(369/371)		 Gly124Arg None ND ND
7.1 GGT>GAG
(239/240)		 Gly80Glu + 7.1.2A +
7.1.3A +
43.7 GAT>GGT
(254),
GGG>AGA
(369/371),
ATA>GTA
(732)		 Asp85Gly,
Gly124Arg,
Ile244Val		 Gln7Arg
(CAA>CGA)		 +++ 43.7.3E +++
43.7.4B +++
9.2 GAT>GGT
(254),
GGG>AGA
(369/371),
ATA>GTA
(732)		 Asp85Gly,
Gly124Arg,
Ile244Val		 +++ 9.2.1A +++
9.2.3B +++
a

Numbering refers to the nucleotide and amino acid positions in the M. mycoides subsp. capri obg gene and protein.

b

None, no TS+ phenotype; +, weak TS+ phenotype; +++, strong TS+ phenotype (as determined by culture assays).

c

ND, not determined.