FIG 6.

Confirmation of specific silencing of RABV. HEK293T cells were transfected with a single siRNA at 25 nM and infected 72 h later with Tha-GFP at an MOI of 5. After 36 h, Hoechst and GFP signals were acquired. Tha-GFP inhibition was determined by comparisons between the negative control (siNEG; 0% inhibition) and the positive control (siGFP; 100% inhibition). GFP% = 100 − [100 × (siHIT − siGFP)/(siCTRL − siGFP)]. Each value represents the mean of data from 3 independent experiments, and error bars represent standard deviations. The statistical significance of results of comparisons of the original siRNA to the corresponding C911 counterpart was determined using ANOVA in Prism 7 (GraphPad). ***, P < 0.001; *, P < 0.05.