Table 2.

Primer sequences, amplicon size and annealing temperature used in Real-time PCR assays

Gene Symbol	Sequence(5′-3′)	Amplicon(bp)	Annealing(°C)
β-actin	GCGGGAAATCGTGCGTGACATT	232	60
	GATGGAGTTGAAGGTAGTTTCGTG
IFNA1	AGAAATACAGCCCTTGTGCC	150	60
	TGACCTGGTGTATGAGTCAATAAG
IFNB1	CAGCTCTTTC CATGAGCTAC	330	60
	CAGCC AGTGC TAGATGAATC
IFNG	GAGATGACTTCGAAAAGCTGAC	149	60
	ACCTCGAAACAGCATCTGAC
H5N1	GACCAATCCTGTCACCTCTGA	251	60
	GTATATGAGGCCCATRCAACT
IFITM1	ATCAACATCCACAGCGAGAC	253	60
	CAGAGCCGAATACCAGTAACAG
IFITM2	TTCATAGCATTCGCGTACTCC	296	60
	GAATACAGGTCAAGGGCAGAG
IFITM3	GAGAACCATCCCAGTAACCC	318	60
	CAACCATCTTCCTGTCCCTAG