Table 1. Primer sequences and primer annealing temperature used on qRT-PCR analysis.

Gene	Sequence	Annealing temperature (°C)
TFF1	FOR: TTTGGAGCAGAGAGGAGGCAATG     REV: ACCACAATTCTGTCTTTCACGGGG	63.3 °C
TFF2	FOR: CCCATAACAGGACGAACTGC     REV: GCACTGATCCGACTCTTGCT	55.7 °C
TFF3	FOR: CTTGCTGTCCTCCAGCTCT     REV: CCGGTTGTTGCACTCCTT	64.5 °C
ACTB	FOR: AAGCACCAGGGCGTGAT     REV: TCGTCCCAGTTGGTGACGA	63.3 °C

TFF1 = trefoil factor family 1 gene; TFF2 = trefoil factor family 2 gene; TFF3 = trefoil factor family 3 gene; ACTB = actin beta gene