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. 2019 May 16;10:1108. doi: 10.3389/fimmu.2019.01108

Figure 3.

Figure 3

Paclitaxel treatment in the presence of an NLRP3 agonist promotes the assembly of the NLRP3 inflammasome. (A) Immunoblots from mouse BMDMs untreated (Unt) or primed with LPS (0.25 μg/mL, 3 h) or paclitaxel (5 μM, 3 h) in the presence of glibenclamide (100 μM) or KCl (30 mM), followed by treatment with nigericin (5 μM, 40 min). Culture supernatants (Sup) or cell lysates (Lys) were immunoblotted with the indicated antibodies. (B) Immunoblots of disuccinimidyl suberate (DSS)-crosslinked pellets (DSS-pel), cellular lysates (Lys) or cultural supernatants (Sup) from mouse BMDMs treated with LPS (0.25 μg/mL, 3 h) or paclitaxel (5 μM, 3 h), followed by treatment with ATP (3 mM, 30 min). (C) Proximity ligation (PL) assay of NLRP3 and ASC in mouse BMDMs treated with paclitaxel (PX, 5 μM, 3 h) or LPS (0.25 μg/mL, 3 h), followed by treatment with ATP (2.5 mM, 30 min). PL signals (red) represent the molecular association of NLRP3 and ASC. Data are shown as a representative image from five-independent samples. Scale bars, 10 μm. (D) Relative percentages of PL signal-positive cells (n = 5). Data are expressed as the mean ± SEM. Asterisks indicate significant differences (*P < 0.05, **P < 0.01).