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. 2019 May 16;10:1108. doi: 10.3389/fimmu.2019.01108

Figure 7.

Figure 7

Paclitaxel promotes IL-1β secretion under physiological conditions. (A,B) Untreated or staurosporine (1 μg/ml, 24 h)-treated A549 cells were washed and incubated with a fresh medium for an additional 18 h to collect culture supernatants. Untreated or paclitaxel (5 μM, 3 h)-pretreated BMDMs were further incubated for 18 h with conditioned medium (A549 culture supernatants + fresh medium, 2:1), and IL-1β or IL-6 levels were assayed in the supernatants by ELISA (n = 3). (C,D) Quantification of IL-1β (C) or IL-6 (D) mRNA levels in BMDMs treated as in (A,B) (n = 3). (E,F) Quantification of IL-1β (D) or IL-6 (E) in extracts of the spleen from Nlrp3+/+ or Nlrp3−/− mice administered the vehicle or paclitaxel (40 mg/kg) for 24 h (n = 3–5). Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001). n.s., not significant.