TABLE 6.
Primer sequences used in the study
| Target or primer | Primer |
Tma (°C) | Amplicon length (bp) | Reference or source | |
|---|---|---|---|---|---|
| Name | Sequence (5′–3′) | ||||
| Bacteria | 27F | AGAGTTTGATCMTGGCTCAG | 53.2 | 1,500 | 64 |
| 1492R | TACGGYTACCTTGTTACGACTT | 54.6 | |||
| 16S rRNA | 784F | RGGATTAGATACCC | 300 | 64 | |
| 1064R | CGACRRCCATGCANCACCT | ||||
| Yeasts | P1F | ATCAATAAGCGGAGGAAAAG | 50.2 | 700 | 65 |
| P2R | CTCTGGCTTCACCCTATTC | 52.4 | |||
| recA gene-based primers | |||||
| L. paraplantarum | ParaF | GTCACAGGCATTACGAAAAC | 51.9 | 107 | 24 |
| L. pentosus | PentF | CAGTGGCGCGGTTGATATC | 55.6 | 218 | 24 |
| L. plantarum | planF | CCGTTTATGCGGAACACCTA | 55.0 | 318 | 24 |
| pREV | TCGGGATTACCAAACATCAC | 52.5 | 24 | ||
| Primers for HRM-qPCR | |||||
| Lactobacillus, Pediococcus, Weissella, Oenococcus | LabF | AGCAGTAGGGAATCTTCCA | 63 | 341 | 69 |
| LabR | CACCGCTACACATGGAG | ||||
| Strain-specific primers for quantification of mahewu isolates in millet malt | |||||
| L. fermentum | CMA1F | CACTAACAGGCACCACTATCTT | 62 | 119 | This study |
| CMA1R | CGCAGTCTTATTCTCATGCTTTAC | ||||
| L. fermentum | CMB1F | AACGCCTAGCCTGATTTATCTC | 62 | 103 | This study |
| CMB1R | CAACAGGATCGTGCCATAGT | ||||
| L. plantarum | CMC1F | AGTTTGCCACATATTAGGAAGAGA | 62 | 112 | This study |
| CMC1R | AGGCTCTAAGGGCTACCTATAC | ||||
a
Tm, melting temperature.