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ATP detected with induced complementation for McpM. E. coli 25 ΔmcpM ΔmcpI with the vector (pMMB207) or with complemented mcpM (pMMB207::mcpM) without IPTG induction showed no detectable ATP in the extracellular environment. Upon induction (0.5 mM IPTG), E. coli 25 ΔmcpM ΔmcpI/pMMB207::mcpM generated elevated levels of ATP (2 to 8 h) compared to the vector-only strain. Results are mean luminescence values for three independent replicates (error bars indicate SEM).
